Section: Research

Postdoctoral Position Available

Gerald Schwarting, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Cell Biology

Other Affiliation(s):
   Program in Neuroscience

Axon Guidance in the Developing Olfactory System

We are investigating the factors that regulate the guidance of connections between sensory neurons in the olfactory epithelium (OE) and their targets (glomeruli) in the olfactory bulb (OB). We have previously demonstrated that neuropilin-1+ (Nnn-1) axons grow exclusively to targets in the medial and lateral OB but not the ventral or dorsal OB. Recently we have recently shown demonstrated that a glycan, Lactosamine also plays a role in axon guidance. The glycosyltransferase b3GnT1 is expressed in neurons in the olfactory epithelium and is the key determinant of Lactosamine expression. It is expressed on the cilia, cell soma and axons on a subset of mature sensory neurons and on targets in the ventral OB, a region where Npn-1 axons are excluded by the repulsive guidance cue Sema3A. In mice lacking b3GnT1, there is a profound axon guidance defect. Axons that normally target the posterior OB are misdirected toward the anterior OB (Figure 1). Interestingly, it has recently been shown that regulation of cAMP and other signaling molecules play a role in axon guidance and are currently investigating the possibility that N-glycosylation by b3GnT1 may play an important role in the expression of key signaling molecules in the OE and OB. These studies suggest that axon guidance is governed by restricted expression of permissive and non-permissive cues that successively restrict subsets of axons into more refined target regions of the developing OB. Our objective is to identify additional guidance mechanisms that further subdivide these targeting compartments into smaller and smaller segments of the OB until precise convergence of axons onto a small number of glomeruli is achieved.

Cell Migration in the Developing Olfactory System

In collaboration with Dr Stuart Tobet and Colorado State University, we are investigating factors that regulate the migration of gonadotropin-releasing hormone (GnRH) neurons. In embryonic mice about 1500 GnRH neurons migrate from the vomeronasal organ (VNO) in the nose across the cribriform plate to the forebrain. The migration of these neurons is essential for reproductive competence in mature mammals, including humans. We have previously shown that GnRH neurons use axons as their guides and that these neurons express a variety of receptors that govern their migration. These axon guides express the netrin-1 receptor deleted in colon cancer (DCC) and turn toward a source of netrin-1 in the ventral forebrain. GnRH neurons normally follow those guides to the ventral forebrain, but in DCC and netrin-1 mutant mice, axons fail to turn ventrally and most GnRH neurons migrate into the cerebral cortex rather than the hypothalamus (see Figure 2). GnRH neurons also express CXCR4, the receptor for stromal cell derived factor 1 (SDF-1), which is expressed in a gradient in the nasal mesenchyme. In CXCR4 null mice, most GnRH neurons fail to migrate out of the nasal mesenchyme during embryonic development. In individuals with Kallmann’s syndrome, GnRH neurons fail to migrate properly but only in a small percentage of cases has a genetic link been identified. Our objectives are to identify additional factors that regulate GnRH neuron migration and to better understand the causes of Kallmann’s and similar syndromes.

Ongoing Projects

Glycoconjugates in Cell-Cell Interactions
Mechanisms of GNRH Neuron Migration During Development

Office: S3-242
Phone: (508) 856-1678
E-mail: Gerald.Schwarting@umassmed.edu
Keywords: Neurobiology, Cell Biology, Developmental Biology

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Postdoctoral Position Available A postdoctoral position is available to study in this laboratory. Contact Dr. Schwarting for additional details.