Section: Rotations

Postdoctoral Position Available

Roger Craig, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Cell Biology

Other Affiliation(s):
   Cell Dynamics Group

Potential Rotation Projects

Project #1: Lipid-Layer Protein Crystallization for Electron Microscopy. While individual protein molecules can be readily visualized by EM, structural information is greatly enhanced if the molecules can by crystallized into 2-dimensional ordered arrays. Methods for achieving this are well established, making use of lipid monolayers at an air-water interface.

1. Follow literature methods to crystallize "standard" proteins using the lipid-layer method.
2. Observe results by electron microscopy following negative staining.
3. If time permits, carry out preliminary image processing of micrographs and/or crystallization of unknown proteins. Techniques to be learned: lipid layer crystallization methods; grid preparation; use of electron microscope; image processing.

Office: S7 210, LAB: S7-209-213
Phone: 508-856-2474
E-mail: Roger.Craig@umassmed.edu
Keywords: Cellular Architecture, Cell Motility, Biophysics, Structural Biology, Imaging and Microscopy

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Postdoctoral Position Available A postdoctoral position is available to study in this laboratory. Contact Dr. Craig for additional details.