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Beckman Spectrophotometer DU 640  Shirwin Pockwinse
Beckman Scintillation Counter LS 6500 Shirwin Pockwinse
Beckman Ultra centrifuge L8-70M Shirwin Pockwinse
Beckman Avanti J-25 centrifuge Shirwin Pockwinse
GE/Amersham Molecular Dynamics Storm Phosphoimager 840 Shirwin Pockwinse
Alpha Innotech Alphaimager 2000 (gel documentation system) Shirwin Pockwinse
Source One Kodak X-Ray Processor M35A with silver recovery system Shirwin Pockwinse
Faxitron  
   
Histology Laboratory with instrumentation for processing and sectioning tissues: Sakura Tissue-Tek Tissue Processor, Sakura Embedding Station, Bright Cryostat, paraffin and plastic embedded tissue microtomes Shirwin Pockwinse
   
Electron Microscopy Facility equipped with state-of-the-art instrumentation Gregory Hendricks

Microscopes:

  • FEI Quanta 200 MKII FEG ESEM; Capable of High Vacuum- High resolution, Environmental, and Low Vacuum Modes for SEM. It is also equipped with an Oxford-Link EDS system for simultaneous elemental analysis allowing the collection of SEI (topographic), BSI (atomic contrast), x-ray data and STEM (transmitted electron imaging) data on most any kind of specimen.
  • Philips CM120 Cryo; with a LaB6 thermionic gun for Cryo-EM and electron tomographic studies. Equipped with a High Resolution Teitz fiber optic coupled digital camera system.
  • Philips CM10-Conventional TEM; with a tungsten thermionic gun for “standard” TEM studies. This microscope is equipped with a fast scan, Gatan lens coupled digital 11 Mega pixel camera system.
  • FEI Tecnai 12 BioTwin; with a LaB6 thermionic gun for Cryo-EM and electron tomographic studies. Equipped with a High Resolution “Eagle” fiber optic coupled digital camera system and a fast scan, Gatan lens coupled digital 11 Mega pixel camera system. 

Ancillary equipment:

  • Ultramicrotomes:
    • 1 Leica EM UC7 room temperature ultramicrotome for sectioning plastic embedded specimens
    • 2 Reichert-Jung UltraCut E ultramicrotomes, and a FC4D cryo chamber. These microtomes can be used for either room temperature sectioning or cryo-sectioning.
  • Leica EM Trim2 plastic block trimmer
  • Denton Vacuum Evaporation system- 502B for making carbon films and metal shadowing
  • Reichert-Jung CS Auto Cryo substitution apparatus for low temperature embedding
  • AutoSamdri-815; critical point dryer for drying specimens for SEM applications
  • Cressington 208HR sputter coater, for producing ultra-thin conductive coatings for High Resolution SEM
  • Balzars Freeze-Fracture apparatus for preparing frozen fractured specimens for TEM analysis of membrane proteins
   
The Confocal Microscopy Core equipped with a Leica SP scanning confocal microscope built around a Leica DMIRBE inverted microscope. This system features 3 lasers (Ar, Kr, and HeNe), spectrophotometric light detection with four channels, a DIC channel, and an Acoustical Optical Tunable Filter. Several perfusion and constant temperature chambers are available for live cell studies. Jeffrey Nickerson or Jean Underwood