Section: Figures

Dario C. Altieri, M.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Cancer Biology

Other Affiliation(s):
   Cancer Center
   Clinical and Population Health Research

Figure 1.  Localization of survivin to the mitotic apparatus.  HeLa cells were stained with monoclonal antibodies (mAb) recognizing immunochemically distinct subcellular pools survivin localized to spindle microtubules (A, mAb 8E2) or kinetochores of metaphase chromosomes (B, mAb 32.1).  In A, image merging is shown of dual labeling for survivin (green) and tubulin (red).

Figure 2.  Cell division defects induced by molecular antagonists of survivin.  HeLa cells were transfected with a survivin antisense oligonucleotide or a survivin Cys84→Ala dominant negative mutant and analyzed by fluorescence microscopy for integrity of the mitotic apparatus (A, B, microtubules), ploidy (C, D, nuclear morphology) and centrosome number (E, F, centrosomes).

Figure 3.  Alternative dimeric structures of mouse survivin.  The three dimer arrangements resolved by X-Ray crystallography of mouse survivin are shown.

Figure 4.  Adenoviral targeting of survivin inhibits tumor growth, in vivo.  Breast cancer xenografts grown in immunocompromised mice were injected with replication-defective adenovirus constructs encoding GFP (pAd-GFP) or a survivin Thr³⁴→Ala dominant negative mutant and analyzed for kinetics of tumor growth (A), or proliferation in situ (B) by Ki-67 staining and immunohistochemistry.

Office: 428
Phone: 508-856-4405
E-mail: Dario.Altieri@umassmed.edu
Keywords: Cell Death, Cancer Biology, Cancer, Checkpoints

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