Section: Publications

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Scot Wolfe, Ph.D.

Academic Role: Associate Professor

Faculty Appointment(s) In:
   Biochemistry and Molecular Pharmacology
   Program in Gene Function and Expression

Other Affiliation(s):
   Interdisciplinary Graduate Program

Publications

Noyes, M.B., Christensen, R.G., Wakabayashi, A., Stormo, G.D., Brodsky, M.H., and Wolfe, S.A. (2008) Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites. Cell, 133: 1277-1289.

Meng, X., Noyes, M.B., Zhu, L.J., Lawson, N.D., and Wolfe, S.A. (2008) Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat. Biotechnol., 26: 695-701.

Meng, X., Thibodeau-Beganny, S., Jiang, T., Joung, J.K., and Wolfe, S.A. (2007) Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method. Nucleic Acids Res., 35: e81.

Meng, X. and Wolfe, S.A. (2006) Identifying DNA sequences recognized by a transcription factor using a bacterial one-hybrid system.  Nature Protoc., 1: 30-45.

Meng, X., Smith, R.M., Giesecke, A.V., Joung, J.K., and Wolfe, S.A. (2006) A counter-selectable marker for bacterial-based interaction trap systems. BioTechniques, 40: 179-184.

Meng, X., Brodsky, M.H., and Wolfe, S.A. (2005) A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nature Biotechnol., 23: 988-994.

Wolfe, S.A., Grant, R.A., and Pabo, C.O. (2003) Structure of a designed dimeric zinc finger protein bound to DNA. Biochemistry, 42: 13401-13409.

Wolfe, S.A., Grant, R.A., Elrod-Erickson, M., and Pabo, C.O. (2001) Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes. Structure, 9: 717-723.

Wolfe, S.A, Ramm, E.I., and Pabo, C.O. (2000) Combining structure-based design with phage display to create new Cys2His2 zinc finger dimers. Structure, 8: 739-750.

Wolfe, S.A., Nekludova, L., and Pabo, C.O. (2000) DNA recognition by Cys2His2 zinc finger proteins. Ann. Rev. Biophys. Biomol. Struct., 29: 183-212.

Wolfe, S.A., Greisman, H.A., Ramm, E.I., and Pabo, C. O. (1999) Analysis of zinc fingers optimized via phage display: evaluating the utility of a recognition code. J. Mol. Biol., 285: 1917-1934.

Pomerantz, J.P., Wolfe, S.A., and Pabo, C.O. (1998) Structure-based design of a dimeric zinc finger protein. Biochemistry, 37: 965-970.

Erlanson, D.A., Wolfe, S.A., Chen, L., and Verdine, G.L. (1997) Selective base-pair destabilization enhances binding of a DNA methyltransferase. Tetrahedron, 53: 12041-12056.

Wolfe, S.A., Zhou, P., Dötsch, V., Chen, L., You, A., Ho, S.H., Crabtree, G.R., Wagner, G., and Verdine, G.L. (1997) Unusual Rel-like architecture in the DNA-binding domain of the transcription factor NFATc. Nature, 385: 172-176.

Wolfe, S.A., Ferentz, A.E., Grantcharova, V., Churchill, M.E.A.,and Verdine, G.L. (1995) Modifying the helical structure of DNA by design: recruitment of an architecture-specific protein to an enforced DNA bend. Chem. Biol., 2: 213-221.

Wolfe, S.A. and Verdine, G.L. (1993) Ratcheting torsional stress in duplex DNA. J. Am. Chem. Soc., 115: 12585-12586.

Office: LRB 619
Phone: 508-856-3953
E-mail: Scot.Wolfe@umassmed.edu
Keywords: Protein-DNA recognition, Gene Therapy, Structural Biology, Biochemistry, Gene Regulation

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