James Evans

Academic Role: Research Associate Professor

Faculty Appointment(s) In:
   Biochemistry and Molecular Pharmacology

Other Affiliation(s):
   Program in Neuroscience

Proteomic Mass Spectrometry Core Labs

Biomedical Applications of Mass Spectrometry

The availability of collaborative resources for the analysis of biological samples by sophisticated instrumental techniques is of immense value for the progress of many diverse biomedical research projects. It is the aim of Mr. Evans' laboratory to meet the mass spectrometry needs of neuroscientists at UMMS. This core Facility provides a high level of expertise and state of the art instrumentation. While the major emphasis of the Facility is on proteomics researchers from a wide variety of disciplines utilize the Facility for all types of mass spectrometry. It provides resources that make research initiatives possible that would otherwise be impossible to carry out. Mr. Evans applies his broad experience in mass spectrometry to solving problems in many biomedical areas including biochemistry, genetics, developmental biology, pharmacology, clinical medicine, physiology, and psychology. Current research in the Facility include development of MS methods for comparative proteomics and protein folding.

Representative Publications

Evans, B.A., Evans, J.E., Baker, S.P., Kane, K., Swearer, J., Hinerfeld, D., Caselli, R., Rogaeva, E., St George-Hyslop, P., Moonis, M., Pollen, D.A.  Long-term statin therapy and csf cholesterol levels: Implications for Alzheimer’s disease.  Dement. Geriatr. Cogn. Disord. 27(6):519-524, 2009.

Orengo, J.M., Leliwa-Sytek, A., Evans, J.E., Evans, B., van de Hoef, D., Nyako, M.,   Day, K., Rodriguez, A. Uric acid is a mediator of the Plamodium falciparum-induced inflammatory response. PLoS One 4(4):e5194, 2009.

Swan, E.E., Peppi, M., Chen, Z., Green, K.M., Evans, J.E., McKenna, M.J., Mescher, M.J., Kujawa, S.G., Sewell, W.F.  Proteomics analysis of perilymph and cerebrospinal fluid in mouse.  Laryngoscope 119(5):953-958, 2009.

Yuan, W., Kang, S.J., Evans, J.E., Cresswell, P.  Natural lipid ligands associated with human CD1d targeted to different subcellular compartments.  J. Immunol 182(8):4784-91, 2009.

Becerra, A., Warke, R.V., Xhaja, K., Evans, B., Evans, J., Martin, K., de Bosch, N., Rothman, A.L., Bosch, I.  Increased activity of indoleamine 2,3-dioxygenase in serum from acutely infected dengue patients linked to gamma interferon antiviral function.  J. Gen. Virol. 90:810-817, 2009.

Godoy, L.C., Munoz-Pinedo, C., Castro, L., Cardaci, S., Schonhoff, C.M., King, M., Tortora, V., Marin, M., Miao, Q., Jiang, J.F., Kapralov, A., Jemmerson, R., Silkstone, G.G., Patel, J.N., Evans, J.E., Wilson, M.T., Green, D.R., Kagan, V.E., Radi, R., Mannick, J.B.  Disruption of the M80-Fe ligation stimulates the translocation of cytochrome c to the cytoplasm and nucleus in nonapoptotoic cells. PNAS Early Edition, #0809279106, 2009.

Co, M.D.T., Orphin, L., Cruz, J., Pazoles, P., Green, K.M., Potts, J., Leporati, A.M., Babon, J.A.B., Evans, J.E., Ennis, F.A., Terajima, M.  In vitro evidence that commercial influenza vaccines are not similar in their ability to activate human T cell responses.  Vaccine 27:319-327, 2009.

Asano, A., Selvaraj, V., Buttke, D.E., Nelson, J.L., Green, K.M., Evans, J.E., Travis, A.J.  Biochemical characterization of membrane fractions in murine sperm: Identification of three distinct sub-types of membrane rafts.  J. Cell. Physiol. 218: 537-548, 2009.

Lam, Y.W., Tam, N.N.C., Evans, J.E., Green, K.M., Zhang, X., Ho, S-M. Differential proteomics in the aging Noble rat ventral prostate. Proteomics Jul;8(13):2750-63, 2008.

Strug, I., Calvo-Calle, J., Green, K., Cruz, J., Ennis, F., Evans, J., Stern, L. Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor. J Proteome Res. Jul;7(7):2703-11. Epub 2008 May 29, 2008.

Orengo, J.M., Evans, J.E., Bettiol, E., Leliwa-Sytek, A., Day K., Rodriguez, A. Plasmodium-induced inflammation by uric acid. PLoS Pathog. Mar 7;4(3):e1000013, 2008.

Godoy, L.C., Pinedo, C., Castro, L., Cardaci, S., Schonhoff, C., King, M., Tortora, V., Marin, M., Miao, Q., Jiang, J., Kapralov, A., Jemmerson, R., Silkstone, G., Patel, J., Evans, J., Wilson, M., Green, D., Kagan, V., Radi, R., Mannick, J.  Translocation of cytochrome C to the cytoplasm and nucleus in non-apoptotic cells.  Free Radical Biol. Med. 43: S31-S32, 2007.

Auclair, J.R., Green, K.M., Shandilya, S., Evans, J.E., Somasundaran, M., Schiffer, C.A.:Mass spectrometry analysis of HIV-1 Vif reveals an increase in ordered structure upon oligomerization in regions necessary for viral infectivity. Proteins 69(2):270-284, 2007.

Liu, L., Zhao, R., Bai, Y., Stanish, L.F., Evans, J.E., Sanderson, M.J., Bonventre, J.V., Rittenhouse, A.R.:M₁ Muscarinic receptors inhibit L-type Ca²⁺ current and M-current by divergent signal transduction cascades.  J. Neurosci. 26(45):11588-11598, 2006.

Morrison, A.R., Moss, J., Stevens, L.A., Evans, J.E., Farrell, C., Merithew, E., Lambright, D.G., Greiner, D.L., Mordes, J.P., Rossini, A.A., Bortell, R.: ART 2, a T cell surface mono-ADP -ribosyltransferase, generates extracellular poly(ADP -ribose).  J. Biol. Chem. 281(44): 33363-72, 2006.

Sullivan, B.D., Evans, J.E., Dana, M.R., Sullivan, D.A.: Influence of aging on the polar and neutral lipid profiles in human meibomian gland secretions.  Arch. Ophthal. 124(9):1286-92, 2006.

Tsai, P.S., Evans, J.E., Green, K.M., Sullivan, R.M., Schaumberg, D.A., Richards, S.M., Dana, M.R., Sullivan, D.A: Proteomic analysis of human meibomian gland secretions. Br. J. Ophthal. 90(3):372-377, 2006.

Sewell, W.F., Mroz, E.A., Evans, J.E., Extracts of retina and brain that excite afferent fibers innervating hair cells contain a compound related to hydroxyphenylglycine-N-carbamoyl. Synapse 58(2): 129-140, 2005.

Calmann, M.A., Evans, J.E., Marinus, M.G. MutS inhibits RecA-mediated strand transfer with methylated DNA substrates. Nucleic Acids Research 33:3591–3597, 2005.

Lam, Y-W., Mobley, J.A., Evans, J.E., Carmody, J., .Ho, S-M.: Mass Profiling-Directed Isolation and Identification of a Stage-Specific Serologic Protein Biomarker of Advanced Prostate Cancer. Proteomics:  5(11):2927-38, 2005. 

Shi, Y., Evans, J.E., Rock, K.: Molecular identification of a danger signal that alerts the immune system to dying cells. Nature, 425:516-521, 2003.

Mobley, J.A., Leav, I., Zielie, P., Wotkowitz, C., Evans, J., Lam, Y., L’Esperance, B.S., Jiang, Z., Ho, S.: Branched fatty acids in dairy and beef products markedly enhance α-methylacyl-CoA racemase expression in prostate cancer cell in vitro. Cancer Epidemiol., Biomarkers & Prevention 12:775-783, 2003.

Academic Background

Ohio State University, 1968
Northeastern University, 1980

Current Position

Director, Proteomics and Mass Spectrometry Facility

Office: Biotech I -- Suite 190
Phone: (508)856-8917
E-mail: James.Evans@umassmed.edu

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