Section: Rotations

Postdoctoral Position Available

Jane Lian, Ph.D.

Academic Role: Professor

Faculty Appointment(s) In:
   Cell Biology

Graduate Student Rotation Projects

Projects are organized for students to develop expertise in an experimental approach and concepts of research design in different areas.

Projects #1 and 2 related to transcriptional control of skeletal development:

Select among the following:

1.    Obtain full length clones and shRNAs from existing libraries to carry out expression studies (knockdown and forced expression), assaying effects on reporter-promoter activity and/or on osteoblast growth and differentiation in vitro.

2.    Protein and mRNA analysis of candidate genes during bone development in vivo or during osteoblast differentiation in vitro and analysis of the target genes for these factors.

3.    Analysis of embryos and tissues from transgenic mice by morphology and molecular approaches.

Project #3 related to cancer cell biology in bone:

Select one:

In vivo molecular imaging of primary tumors through metastatic sites.

Characterization of signaling pathways that activate metastatic genes regulated by Runx2 in cancer cells.

Knock down of the Runx2 transcription factor by shRNA technology in metastatic cells combined with in vitro and in vivo testing of invasive and metastatic properties.

Project #4 related to stem cell biology and tissue engineering:

Select one:

In vitro differentiation of human stem cell derived embryoid bodies into cartilage and bone by BMP2 and Wnt signaling. 

Isolation of mouse embryo fibroblasts or marrow stem cells from mouse models with bone phenotypes for characterizing their defects.

Augmentation of fracture repair in mouse models of skeletal diseases using stem cells.

Office: S3-326
Phone: 508-856-5941
E-mail: Jane.Lian@umassmed.edu
Keywords: Skeletal Biology, Stem Cell Biology, Mouse Genetics, Metastasis, Gene Regulation

More on Jane Lian's Research Research | Figures | Publications | Rotations | Biography View All Sections on One Page

Postdoctoral Position Available Postdoctoral Fellowship Positions Open Preference is given to applicants with experience in nucleic acid chemistry and chromatin, molecular approaches and with interest in in vivo genetic studies, cell growth and differentiation and stem cell biology for application to skeletal related problems and cancer metastasis.