SOLiD_4 Library Preparation 

Please NOTE: You need to construct the library through the addition of the P1 and P2 sequences, gel purify, quantify, and validate by TOPO Cloning and sequencing 20 or so single clones as a representative population sample. The Core Lab Staff will perform the subsequent steps, i.e. the "SOLiD™ System templated bead preparation and sequencing"

Note:  ABI and Ambion Kits and Reagents are available through the MBCL (Reagent Program)

Current Kits/Protocols/Possibly Useful Stuff From SOLiD High Council
SOLiD Total RNA Seq (STaR) 
    Whole Transcriptome
    Small RNA
    Alternative Fragmentation

DNA Based Protocols
       ChIP-Seq
       SNP
       Whole Genome
       Mate-Pair

BarCode Information, Primer Sequences
        Coming soon

User Submitted SOLiD4 Protocols, Tips, Info
  (be the first, win a valuable prize .. or TShirt)

These are the "old" SOLiD 3 Protocols

ABI Official Whole Transcriptome Library Protocol  WTAK

ABI Official Small RNA Library Protocol   SREK 
(notes: adapter set A works better, refer to color balance chart when chosing barcode numbers)

DeepSeq Core Lab Fragment Library Protocol
Based on the ABI library preparation method       FRG_1109_DW