Molecular Biology Core Labs  ~ MBCL ~
     Open business days 9:00 am - 5:00 pm
     In case of extreme weather (bad or good), deliveries may be postponed
     If UMassMed is on "inclement weather alert", we're closed
     
The MBCL offers several services and reagent programs to UMass Labs*
Email us for services and pricing information at  MBCL@umassmed.edu

CHANGE IN DROP OFF LOCATIONS, School 5th floor spot has moved to Sherman 8th floor!
New Fridge Location is ASC 8th Floor, Room 2058 (mail room)

Oligonucleotide Program
The MBCL offers discounted DNA, RNA, and specialty oligos to UMassMed Labs
Please email us for a pricing list. To order online or to download forms for large orders or 96 well plate oligo preparations, please go to http://www.umassmed.edu/OLIGO

DNA Sequencing  (for information about viewing and editing sequences, click here) 

Standard DNA Sequencing  The user provides template(s) and custom primers or specifies a standard primer (list).  This program includes diluting and mixing templates with primers, custom sequencing protocols, and special reaction conditions.  Use this form (Click here for Standard Reaction + Analysis ticket)

EconoSEQ   The Economy sequencing option is available for users who set up their reactions (template+primer) in the presecribed format. These are processed on the HTP platform at a lower price than standard sequencing. Includes reaction and analysis (Click here to download ticket with info).   
note this is a three page excel document, use the tabs to change forms
THERE IS AN AREA ON THE TICKET FOR YOUR NAME & PHONE NUMBER PLEASE BE SURE TO FILL IT OUT 
  Click here to download JUST THE TICKET 96 Well-Plate Version 
  Click here to download JUST THE TICKET StripTubes Version
  We have 3 drop off locations (see bottom of this page)

HTP (High Thru-Put)  Pricing is per 96 well plate and depends on Project Size, for single plate pricing or a large scale quote, contact us at MBCL@umassmed.edu

BioAnalyzer  The MBCL operates a BioAnalyzer service using the Agilent Lab-on-chip system.  Samples are analyzed for concentration and molecular weight/size, small RNA, total RNA and DNA analysis are available. Contact the core to schedule an analysis or if you have questions BioAnalyzer. ( Order Ticket UMASS June 2012 ) Note pricing of the High Sensitivity DNA Chip will increase on July 1st. The BioAnalyzer Service is for the analysis of RNA and DNA samples submitted by investigators. If you are interested in QC of your Deep Sequencing Core Lab sample, please submit them to the DSCL.

BIACore SPR Workstation The MBCL maintains a BIACoreX SPR workstation for molecular interaction analyses. Please contact us to schedule training or use of the instrument MBCL@umassmed.edu

Next-Generation (DEEP) Sequencing or NGS or MPSS
Please visit the web site for information about, NEMO and KRAKEN our Illumina GA2 Genome Analyzers and a link to a web-video about color-space and our new SOLiD 4 Analyzer, THE BEAGLE.
http://www.umassmed.edu/nemo 

There are 3 drop-off locations for samples (Sequencing, Bioanalyzer, etc)
        2 Biotech, across from room 207 (pick up at 12:30pm)
        LRB, 6th Floor Coat Room 660 (pick up at 11:00am)
        Sherman Bldg, ASC8-2058, 8th Floor, Mail Room (pick up at 11:00am)
Please read the instructions and use the stickers and bags provided.
*** If you are sending your samples (or data drive) to MBCL, please contact us and verify the address. Samples sent to the wrong address are not the responsibility of the core lab.

* Other institutions and research groups are eligible to use many of our services. However pricing may vary. Off-campus users should contact us for a quote ( Ellen.Kittler@umassmed.edu )
 
For a letter of support for a research grant or project, please provide the same format and information as requested for DeepSeq support letters (click here).
For a list of Frequently Asked Questions, and the answers, click here
Select RESOURCES in the left nav for info on free sequence view/edit software