Click links for PDFs of Publications.


Bossé GD, Rüegger S, Ow MC, Vasquez-Rifo A, Rondeau EL, Ambros VR, Großhans H, and Simard MJ. (2013) The Decapping Scavenger Enzyme DCS-1 Controls MicroRNA Levels in Caenorhabditis elegans. Molecular Cell. 2013 Mar 26. doi:pii: S1097-2765(13)00179-2. 10.1016/j.molcel.2013.02.023. [Epub ahead of print]

Zou Y, Chiu H, Zinovyeva A, Ambros V, Chuang C-F, Chang C. (2013) Developmental decline in neuronal regeneration by the progressive change of two intrinsic timers. Science. 2013 Apr 19;340(6130):372-376


Karp X, Ambros V. (2012) Dauer larva quiescence alters the circuitry of microRNA pathways regulating cell fate progression in C. elegans. Development. 2012 Jun; 139(12):2177-86.


Ambros V. (2011) MicroRNAs and developmental timing. Curr Opin Genet Dev. 2011 Apr 28 apdfIcon

Karp, X., Hammell, M., Ow, M.C., and Ambros, V. (2011) Effect of life history on microRNA expression during C. elegans development. Apr;17(4):639-51. Epub 2011 Feb 22. apdfIconPMID: 21343388.

Karp, X., Ambros, V. (2011) The developmental timing regulator hbl-1 modulates the dauer formation decision in Caenorhabditis elegans. Genetics. 2011 Jan;187(1):345-53 apdfIcon


Zheng G, Ambros V, Li WH. (2010) Inhibiting miRNA in Caenorhabditis elegans using a potent and selective antisense reagent. Silence. 2010 Apr 1;1(1):9.PMID: 20359322 apdfIcon


Ambros V. (2009). pRB/CKI pathways at the interface of cell cycle and development. Cell Cycle. 2009 Nov 16;8(21) apdfIcon

Hammell, C.M., Karp, X. and Ambros, V. (2009) A feedback circuit involving let-7-Family miRNAs and DAF-12 integrates environmental signals and developmental timing in C. elegans. Proc Natl Acad Sci U S A. 2009 Oct 13 apdfIcon

Hong, X., Hammell, M., Ambros, V., and Cohen, S.M., (2009). Identification of microRNA targets by immunopurification of Ago-1 RNPs: selection for a distinct class of targets. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):15085-90 apdfIcon

Zhang, L., Hammell, M., Kudlow, B.A., Ambros, V. and Han, M. (2009) Systematic analysis of dynamic miRNA-target interactions during C. elegans development. Development. 2009 Sep;136(18):3043-55 apdfIcon

Hammell, C.M., Lubin, I., Boag, P.R., Blackwell, K.T., Ambros, V. (2009) nhl-2 modulates miRNA activity in Caenorhabditis elegans. Cell. 2009 Mar 6;136(5):926-38. apdfIcon


Ow MC, Martinez NJ, Olsen PH, Silverman HS, Barrasa MI, Conradt B, Walhout AJ, Ambros V. The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic expression of microRNA genes in C. elegans. Genes Dev. 2008 Sep 15;22(18):2520-34. PMID 18794349 apdfIcon

Martinez, N. J., Ow, M. C., Barrasa, M. I., Hammell, M., Sequerra, R., Doucette-Stamm, L., Roth, F. P., Ambros, V. R., Walhout, A. J. M. (2008). A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity. Genes and Devel. Sep 15;22(18):2535-49 apdfIcon

Hammell, M., Long, D., Zhang, L., Lee, A., Carmack, C. S., Han, M., Ding, Y. and Victor Ambros, V. (2008). mirWIP: microRNA target prediction based on miRNP enriched transcripts. Nature Methods. Sep: 5(9): 813-819 apdfIcon

Sokol NS, Xu P, Jan YN, Ambros V. Drosophila let-7 microRNA is required for remodeling of the neuromusculature during metamorphosis. Genes Dev. 2008 Jun 15;22(12):1591-6. PMID 18559475 apdfIcon

Martinez, N. J., Ow, M. C., Reece-Hoyes, J., Barrasa, M. I, Ambros, V. R., Walhout, A. J. M. (2008). Comprehensive spatio-temporal analysis of Caenorhabditis elegans microRNA expression Genome Research 18(12):2005-15 apdfIcon


Ambros, V. and Chen, C. (2007). The regulation of genes and genomes by small RNAs Development, May: 1 34(9):1635-41 apdfIcon

Miska, E. A., Alvarez-Saavedra, E., Abbott, A. L., Lau, N. C., Hellman, A. B., McGonagle, S. M., Bartel, D. P., Ambros, V.R. and Horvitz, H. R. (2007) Most Caenorhabditis elegans microRNAs are individually not essential for development or viability. PLoS Genet. 2007 Dec 14;3(12):e215 

Gaur, A.B. , Jewell, D.A., Liang, Y., Ridzon, D. , Moore, J.H. Chen, C., Ambros,V. R. and Israel, M. A. (2007). Characterization of microRNA expression levels and their biological correlates in human cancer cell lines. Cancer Res 67: (6). 2456-2468 apdfIcon

Long, D., Lee, R., Williams, P., Chan, C.Y., Ambros,V. and Ding, Y. (2007). Potent Effect of Target Structure in MicroRNA Function. Nature Structure Mol Biol. 14; 287-294 apdfIcon

Hinas, A, Reimegård, J. Wagner, E.G.H., Nellen, W., Ambros, V.R. and Söderbom, F. (2007) The small RNA repertoire of the unicellular amoeba Dictyostelium discoideum: microRNA candidates, small antisense RNAs that may be derived from longer transcripts and multiple classes of repeat-associated RNAs. Nucleic Acids Research 2007;35(20):6714-26


Lee RC, Hammell CM, Ambros V. Interacting endogenous and exogenous RNAi pathways in Caenorhabditis elegans. RNA. 2006 Apr;12(4):589-97. Epub 2006 Feb 17. PMID 16489184 apdfIcon


Karp, X. and Ambros, V. (2005) Encountering microRNAs in cell fate signalling Science 310(5752): 1288 - 1289 apdfIcon

Sokol NS, Ambros V. Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. Genes Dev. 2005 Oct 1;19(19):2343-54. Epub 2005 Sep 15. PMID 16166373 apdfIcon

Abbott AL, Alvarez-Saavedra E, Miska EA, Lau NC, Bartel DP, Horvitz HR, Ambros V. The let-7 MicroRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans. Dev Cell. 2005 Sep;9(3):403-14. PMID 16139228 apdfIcon

Kuhlmann, M., Borisova, B. E., Kaller, M., Larsson, P., Stach, D., Na, J. Eichinger, L., Lyko, F., Ambros, V., Söderbom, F., Hammann, C. and Nellen, W. (2005) Silencing of retrotransposons in Dictyostelium by DNA methylation and RNAi Nucl. Acids Res. Nov 10;33(19):6405-1 apdfIcon

Hristova, M., Birse, D., Hong, Y. and Ambros, V. (2005) The Caenorhabditis elegans Heterochronic Regulator LIN-14 Is a Novel Transcription Factor That Controls the Developmental Timing of Transcription from the Insulin/Insulin-Like Growth Factor Gene ins-33 by Direct DNA Binding Mol Cell Biol. 2005 (24):11059-72 apdfIcon


Lee, R. C., Feinbaum, R. and Ambros, V. (2004). A short History of a Short RNA Cell, Vol. S116, S89–S92 apdfIcon

Ambros, V. (2004). The functions of animal microRNAs Nature (Insight) 431, 350 – 355 apdfIcon

Pepper AS, McCane JE, Kemper K, Yeung DA, Lee RC, Ambros V, Moss EG. The C. elegans heterochronic gene lin-46 affects developmental timing at two larval stages and encodes a relative of the scaffolding protein gephyrin. Development. 2004 May;131(9):2049-59. Epub 2004 Apr 8. PMID 15073154 apdfIcon

Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V. Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol. 2004;5(3):R13. Epub 2004 Feb 16. PMID 15003116 apdfIcon


Ambros, V., and Lee, R. (2003) Identification of microRNAs and other tiny noncoding RNAs by cDNA cloning in RNA Interference, Editing, and Modification, Methods and Protocols , J.Gott ,Ed 

Carrington, J., Ambros, V. (2003) Roles of Small RNAs in Development of Plants and Animals. Science 301 July 18(5631):336-8apdfIcon

Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V. Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-Complex gene activity. Dev Biol. 2003 Jul 1;259(1):9-18. PMID 12812784 apdfIcon

Ambros V, Lee RC, Lavanway A, Williams PT, Jewell D. MicroRNAs and other tiny endogenous RNAs in C. elegans. Curr Biol. 2003 May 13;13(10):807-18. PMID 12747828 apdfIcon

Ambros V. (2003). MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell.113, 673-676

Ambros V, Bartel B, Bartel DP, Burge CB, Carrington JC, Chen X, Dreyfuss G, Eddy SR, Griffiths-Jones S, Marshall M, Matzke M, Ruvkun G, Tuschl T (2003). A uniform system for microRNA annotation. RNA 9, 277-279


Sempere LF, Dubrovsky EB, Dubrovskaya VA, Berger EM, Ambros V. The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis in Drosophila melanogaster. Dev Biol. 2002 Apr 1;244(1):170-9. PMID 11900466 apdfIcon


Ambros, V. (2001) Dicing up RNAs. Science (Perspectives) Aug 3;293(5531):811-3

Ambros, V. (2001) MicroRNAs: Tiny regulators with great potential. Cell 107, 823-826

Lee RC, Ambros V. An extensive class of small RNAs in Caenorhabditis elegans. Science. 2001 Oct 26;294(5543):862-4. PMID 11679672


Ambros, V. (2000) Control of developmental timing in Caenorhabditis elegans. Current Opinions in Development and Genetics 10, 428-433

Ambros, V. (2000) The temporal control of cell cycle and cell fate in Caenorhabditis elegans. In Novartis Symposium on The Cell Cycle in Development, Novartis Foundation P. Nurse, Ed

Hong Y, Lee RC, Ambros V. Structure and function analysis of LIN-14, a temporal regulator of postembryonic developmental events in Caenorhabditis elegans. Mol Cell Biol. 2000 Mar;20(6):2285-95. PMID 10688674 apdfIcon

Slack, F. J., Basson, M., Liu, Z., Ambros, V., Horvitz, H. R. and Ruvkun, G. (2000) The lin-41 RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor. Molecular Cell 5, 659-669 apdfIcon


Olsen PH, Ambros V. The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation. Dev Biol. 1999 Dec 15;216(2):671-80. PMID 10642801 apdfIcon

Feinbaum R, Ambros V. The timing of lin-4 RNA accumulation controls the timing of postembryonic developmental events in Caenorhabditis elegans. Dev Biol. 1999 Jun 1;210(1):87-95. PMID 10364429 apdfIcon

Ambros V. Cell cycle-dependent sequencing of cell fate decisions in Caenorhabditis elegans vulva precursor cells. Development. 1999 May;126(9):1947-56. PMID 10101128 apdfIcon


Hong Y, Roy R, Ambros V. Developmental regulation of a cyclin-dependent kinase inhibitor controls postembryonic cell cycle progression in Caenorhabditis elegans Development. 1998 Sep;125(18):3585-97. PMID 9716524 apdfIcon


Ambros, V. R. (1997) Heterochronic genes. In Caenorhabditis elegans. D. Riddle, Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 

Moss EG, Lee RC, Ambros V. The cold shock domain protein LIN-28 controls developmental timing in C. elegans and is regulated by the lin-4 RNA. Cell. 1997 Mar 7;88(5):637-46. PMID 9054503 apdfIcon


Euling S, Ambros V. Reversal of cell fate determination in Caenorhabditis elegans vulval development. Development. 1996 Aug;122(8):2507-15. PMID 8756295 apdfIcon

Euling S, Ambros V. Heterochronic genes control cell cycle progress and developmental competence of C. elegans vulva precursor cells. Cell. 1996 Mar 8;84(5):667-76. PMID 8625405 apdfIcon


Liu, Z., Kirch, S., Ambros, V. R. (1995) The C. elegans heterochronic gene pathway controls the stage-specific transcription of collagen genes. Development, 121, 2471-2478 apdfIcon

Rougvie, A., Ambros, V. R. (1995) The lin-29 locus of C. elegans encodes a Zn-finger protein that regulates a stage-specific transcriptional switch. Development, 121, 2491-2500 apdfIcon


Ambros, V., and Moss, E. (1994) The heterochronic genes and developmental timing in C. elegans. Trends in Genetics, 10, 123-127

Ambros, V. Genetic basis for heterochronic variation" in Heterochrony in Evolution, Michael L. McKinney, Ed. Plenum, New York 


Lee, R., Feinbaum, R., Ambros, V. (1993) The heterochronic gene lin-4 of C. elegans encodes small RNAs with antisense complementarity to lin-14. Cell, 75, 843-854 apdfIcon


Papp A, Rougvie AE, Ambros V. (1991) Molecular cloning of lin-29, a heterochronic gene required for the differentiation of hypodermal cells and the cessation of molting in C.elegans. Nucleic Acids Res. Feb 11;19(3):623-30

Liu, Z and Ambos, V (1991). Alternative temporal control systems for hypodermal cell differentiation in Caenorhabditis elegans. Nature 350, 162-165

Mello CC, Kramer JM, Stinchcomb D, Ambros V. (1991) Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. Dec;10(12):3959-70 apdfIcon


Liu Z.C., Ambros V. (1989) Heterochronic genes control the stage-specific initiation and expression of the dauer larva developmental program in Caenorhabditis elegans. Genes Dev. Dec;3(12B):2039-49 apdfIcon

Hodgkin J, Papp A, Pulak R, Ambros V, Anderson P. (1989) A new kind of informational suppression in the nematode Caenorhabditis elegans. Genetics. Oct;123(2):301-13apdfIcon

Ruvkun G, Ambros V, Coulson A, Waterston R, Sulston J, Horvitz HR.(1989) Molecular genetics of the Caenorhabditis elegans heterochronic gene lin-14. Genetics. Mar;121(3):501-516

Ambros V. (1989) An hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell. Apr 7;57(1):49-57 


Ambros V, Horvitz HR. (1987) The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic developmental events. Genes Dev. Jun;1(4):398-414


Ambros V, Horvitz HR. (1984) Heterochronic mutants of the nematode Caenorhabditis elegans. Science. Oct 26;226 (4673):409-16


Ambros V, Baltimore D. (1980) Purification and properties of a HeLa cell enzyme able to remove the 5'-terminal protein from poliovirus RNA. J Biol Chem. Jul 25;255(14):6739-44


Ambros V, Pettersson RF, Baltimore D. (1978) An enzymatic activity in uninfected cells that cleaves the linkage between poliovirion RNA and the 5' terminal protein. Cell. Dec;15(4):1439-46

Pettersson RF, Ambros V, Baltimore D.(1978) Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA. J Virol Aug;27(2):357-65

Ambros V, Baltimore D. (1978) Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine. J Biol Chem Aug 10;253 (15):5263-6


Hewlett MJ, Rozenblatt S, Ambros V, Baltimore D. (1977) Separation and quantitation of intracellular forms of poliovirus RNA by agarose gel electrophoresis Biochemistry. Jun 14;16 (12):2763-7

Flanegan JB, Pettersson RF, Ambros V, Hewlett NJ, Baltimore D. (1977) Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus. Proc Natl Acad Sci U S A. Mar; 74 (3): 961-5.


Ambros VR, Chen LB, Buchanan JM (1975). Surface ruffles as markers for studies of cell transformation by Rous sarcoma virus. Proc Natl Acad Sci U S A. Aug; 72(8):3144-8 apdfIcon


Gruberg ER, Ambros VR. (1974) A forebrain visual projection in the frog (Rana pipiens). Exp Neurol. Aug; 44(2):187-197