Protocols

The following protocols pertain to lentiviral vectors (pGIPZ and TRC). If you wish to obtain protocols for the retroviral vector (pSM2), please contact the RNAi Core Facility via email at shrnaclone@umassmed.edu.

Protocol for producing lentiviral (pGIPZ or TRC) supernatant.  Viral Supernant Production Protocol

Protocol for infecting cells with lentiviral (pGIPZ or TRC) supernatant.  Infection Protocol