Instrumentation

 LTQ_Velos_ProThermo Scientific LTQ Orbitrap Velos Pro with Proxeon EasyLC
A linear ion trap-Orbitrap hybrid mass spectrometer employing CID, HCD, and ETD fragmentation techniques for analysis of complex protein digests or top-down whole protein analysis. Identification of post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination and chemical modifications including protein-drug adducts and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC) or label-free quantitative proteomics approaches.
 
Q_Exactive Thermo Scientific Q-Exactive with Waters NanoAcquity UPLC or Thermo Accela 1250 UPLC
A quadrupole-Orbitrap hybrid mass spectrometer employing HCD fragmentation for the analysis of complex protein digests, small molecule profiling/quantitation, or whole protein analysis. Identification of post-translational modifications and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC, TMT, iTRAQ) or label-free quantitative proteomics approaches. Nanoflow (NanoAcquity) and conventional flow (Accela) UPLC allow for a wide range of chromatographic applications.
 
Agilent_QToF Agilent Technologies 6520 Q-TOF with Infinity 1260 HPLC
A quadrupole time-of-flight mass spectrometer for metabolomic and small molecule quantitative applications.
 
Quattro_Premier Waters Quattro Premier XE triple quadrupole with Waters Acquity UPLC or Waters NanoAquity UPLC
A triple quadrupole mass spectrometer for quantitation of small molecules and peptides. Conventional flow (Acquity) and nanoflow (NanoAcquity) UPLC allow for a wide range of chromatographic applications.
 
TOF2_shimadzu Shimadzu Axima TOF2 (TOF-TOF)
A MALDI time-of-flight tandem instrument for high energy CID analysis of whole proteins, peptides, polymers, carbohydrates, and glycolipids. Top-down protein sequencing by in-source decay, peptide de novo sequencing by post-source decay.
 
QTOF_Waters Waters Q-TOF Premier with Advion Triversa Nanomate or HDX MS interface
A quadrupole time-of-flight hybrid mass spectrometer for autmated (Nanomate) exact mass measurment of small molecules or whole proteins. Hydrogen-deuterium (H/D) exchange protein confirmation studies using an online pepsin HDX interface coupled to a CID MSE data-acquisition platform.
 
LTQ Thermo Scientific LTQ with Advion Triversa NanoMate or Thermo Surveyor HPLC
A linear ion trap mass spectrometer for the analysis of small molecules introducted by automated infusion (Nanomate) or HPLC (Surveyor).

Proteomics and Mass Spectromy Facility
UMass Medical School

Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
Inquiries

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